Review
doi: 10.1016/j.cbpa.2005.09.018.
Epub 2005 Oct 13.
Protein semisynthesis and expressed protein ligation: chasing a protein's tail
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- PMID: 16226484
- DOI: 10.1016/j.cbpa.2005.09.018
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Review
Protein semisynthesis and expressed protein ligation: chasing a protein's tail
Curr Opin Chem Biol.
2005 Dec.
Abstract
The adaptation of native chemical ligation to protein semisynthesis has become a powerful way to address problems in the analysis of protein structure and function. In particular, the exploitation of nature's inteins in expressed protein ligation is now a standard approach in the study of proteins. Site-specific incorporation of unnatural amino acids, biophysical probes and post-translational modifications in proteins have led to new insights into enzyme mechanisms, protein folding, ion channel function, translation and signaling.
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