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Case Reports
. 2020 Sep 12;5(12):2365-2370.
doi: 10.1016/j.ekir.2020.09.011. eCollection 2020 Dec.

Anti-LRP2 Nephropathy

Alexander J Gallan  1 , Arvind Garg  2 , A Bernard Collins  3 , Laurence H Beck Jr  4 , Claire Trivin-Avillach  4 , Kammi J Henriksen  5 , Anthony Chang  5
Affiliations
Case Reports

Anti-LRP2 Nephropathy

Alexander J Gallan et al. Kidney Int Rep. .
No abstract available

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Figures

Figure 1
Figure 1
(a) On low power, the kidney biopsy showed extensive attenuation of the proximal tubules with loss of the brush borders, patchy mild interstitial inflammation, thickening of the tubular basement membranes, and diffuse and frequently marked interstitial fibrosis and tubular atrophy (periodic acid–Schiff [PAS] stain, original magnification ×100). (b) On higher power, the thickened proximal tubular basement membranes resemble atrophic changes, but exhibit an atypical “smudgy” appearance. The proximal tubular epithelial cells demonstrate severe tubular injury with loss of brush borders (PAS stain, original magnification ×400).
Figure 2
Figure 2
(a) The proximal tubular basement membranes exhibit diffuse “smudgy” immunofluorescence staining for IgG. (b) A few glomeruli showed segmental granular capillary wall immunofluorescence staining for IgG. (c) Conspicuous electron-dense deposits were present in the proximal tubular basement membranes by electron microscopy. (d) Segmental subepithelial electron-dense deposits with intervening glomerular basement membrane “spikes” were present by electron microscopy.
Figure 3
Figure 3
(a) Indirect immunofluorescence shows the patient’s serum staining the renal tubular brush border in a frozen section of normal human kidney (×100). (b–d) Colocalization of the patient’s serum IgG (b, green) and anti-LRP2 (c, red) double stained by multistep indirect immunofluorescence on frozen sections of normal human kidney tissue. Confocal microscopy of merged image demonstrates colocalization of IgG and LRP2 (d, orange).
Figure 4
Figure 4
Immunoprecipitate (IP) with human tubular extract (HTE) and patient serum (lane 1), positive ABBA serum (lane 2), and normal human serum (lane 3), run on polyacrylamide gel electrophoresis and blotted for anti-LRP2. Both patient serum and positive control pulled down LRP2 from HTE compared with normal human serum.
See this image and copyright information in PMC

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References

    1. Raghavan R., Eknoyan G. Acute interstitial nephritis - a reappraisal and update. Clin Nephrol. 2014;82:149–162. - PMC - PubMed
    1. Park M.H., D'Agati V., Appel G.B. Tubulointerstitial disease in lupus nephritis:relationship to immune deposits, interstitial inflammation, glomerular changes, renal function, and prognosis. Nephron. 1986;44:309–319. - PubMed
    1. Rosales I.A., Collins A.B., do Carmo P.A. Immune Complex tubulointerstitial nephritis due to autoantibodies to the proximal tubule brush border. J Am Soc Nephrol. 2016;27:380–384. - PMC - PubMed
    1. Larsen C.P., Trivin-Avillach C., Coles P. LDL receptor-related protein 2 (megalin) as a target antigen in human kidney anti-brush border antibody disease. J Am Soc Nephrol. 2018;29:644–653. - PMC - PubMed
    1. Cornell L.D. IgG4-related kidney disease. Curr Opin Nephrol Hypertens. 2012;21:279–288. - PubMed

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