Materials and Methods Hemoglobin solutions were prepared from freshly drawn human
blood by the method of Drabkin (13)) exhaustively dialyzed against distilled water in boiled
Visking casing, and centrifuged at high speed before use. The oxyhemoglobin concentration
was determined spectrophotometrically, with a molar extinction coefficient of 5.6 x lo4 at 540
mp and 5.9 X lo4 at 576 rnp. Solutions were discarded when the ratio of the extinctions at
576/540 rnp fell below 1.05. Nitrogen was “prepurified nitrogen,” purity 99.9%. The sources�…

The hemoglobin H used for these studies was obtained from two patients. The first patient,
LG, was a 25-year-old woman of Negro and American Indian ancestry. The second patient,
NH, was a 51-year-old woman of Chinese ancestry. The evidence that the hemoglobin H of
both of these patients is identical with hemoglobin H studied by others is summarized below.
1. Patient LG had a moderate microcytic hypochromic anemia; after incubation with brilliant
cresyl blue, the erythrocytes showed characteristic inclusion bodies (2). Patient NH had�…