A DNA barcode for land plants
- PMID: 19666622
- PMCID: PMC2722355
- DOI: 10.1073/pnas.0905845106
A DNA barcode for land plants
Abstract
DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF-atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK-psbI spacer, and trnH-psbA spacer). Based on assessments of recoverability, sequence quality, and levels of species discrimination, we recommend the 2-locus combination of rbcL+matK as the plant barcode. This core 2-locus barcode will provide a universal framework for the routine use of DNA sequence data to identify specimens and contribute toward the discovery of overlooked species of land plants.
Conflict of interest statement
Conflict of interest statement: Following the publication of Lahaye et al. (PNAS 105:2923, 2008), the process of filing a patent on DNA barcoding of land plants using
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Comment in
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A botanical macroscope.Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):12569-70. doi: 10.1073/pnas.0906757106. Epub 2009 Aug 3. Proc Natl Acad Sci U S A. 2009. PMID: 19666620 Free PMC article. No abstract available.
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