二氢硫辛酰胺脱氢酶(EC 1.8.1.4,英語:Dihydrolipoamide dehydrogenase,缩写DLD,又称为线粒体二氢硫辛酸脱氢酶,dihydrolipoyl dehydrogenase, mitochondrial)是一种由人类基因DLD[1][2][3][4]所编码的黄素蛋白,其作用是将二氢硫辛酰胺脱氢从而转化为氧化型硫辛酰胺。
DLD作为一种线粒体蛋白质,在真核生物的能量代谢中起到重要作用,它至少参与了五种多酶复合体,且为复合体完成反应所必需的组份[5]。另外,DLD作为一种黄素蛋白氧化還原酶,以FAD为辅基接受质子与电子催化二硫键的形成。
DLD是大小为51千道尔顿亚基的同二聚体,其中每个亚基都与一分子的FAD以共价键的形式相连[6]。
在哺乳动物的线粒体三羧酸循环中,该酶与丙酮酸脱氢酶和二氢硫辛酰基乙酰基转基酶一起组成中丙酮酸脱氢酶复合体,同时该酶亦是α-酮戊二酸脱氢酶复合体中的一个组分。二氢硫辛酰胺脱氢酶的主要任务是负责将复合物中另一组分硫辛酰胺转乙酰基酶(或二氢硫辛酰琥珀酰转移酶)的辅基二氢硫辛酰胺脱氢从而转化为氧化型硫辛酰胺,该酶也是线粒体甘氨酸剪切体系的组分。
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二氢硫辛酰胺脱氢酶引用了美国国家医学图书馆提供的資料,这些資料属于公共领域。